“elisa-cief” Using Capillary-based Microdevice: Highly-sensitive Elisa Based on Capillary-isoelectric Focusing of Enzyme Reaction Product
نویسندگان
چکیده
Sensitivity enhancement of enzyme-linked immunosorbent assay (ELISA) in capillary-based microdevice was achieved by the concentration of fluorescent final enzyme reaction product using capillary-isoelectric focusing (CIEF). In order to realize this, fluorescent substrate molecule, which can change its structure into ampholyte ion after an enzyme reaction, was newly synthesized and used. Approximately seven-fold enhancement of sensitivity and 1-2 orders of magnitude lower the detection limit were achieved.
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